Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis / Analiza genetske variabilnosti akcesij grahorja (Lathyrus inconspicuous (L.) s SDS poliakrilamidno gelsko elektroforezo

SDS Polyacrylamide Gel Electrophoresis of Proteins.

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.

Genetic diversity among induced mutants of grasspea (Lathyrus sativus L.)

Introduction Knowledge about genetic diversity is a prerequisite of any breeding programme. Inclusion of diverse parents in hybridisation programmes serves the purpose of combining desirable genes in new recombinations. Mahalanobi’s D statistic (7) is a powerful tool in quantifying the degree of divergence at genotypic level. Several workers have used this method to quantify the degree of diver...

Characterization of Bothrops Venoms of Argentina by Sds-polyacrylamide Gel Electrophoresis

Electrophoretic pattern of Bothrops alternatus, B.neuwiedi, B. ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (including juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue fluids. All the snak...

Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recove...